Biochemical, Mechanistic, and Spectroscopic Characterization of Metallo-β-lactamase VIM-2

@inproceedings{Aitha2014BiochemicalMA,
  title={Biochemical, Mechanistic, and Spectroscopic Characterization
of Metallo-β-lactamase VIM-2},
  author={Mahesh Aitha and Amy R. Marts and Alex Bergstrom and Abraham Jon M\oller and Lindsay Moritz and Lucien Turner and Jay C. Nix and Robert A. Bonomo and Richard C Page and David L Tierney and Michael W Crowder},
  booktitle={Biochemistry},
  year={2014}
}
This study examines metal binding to metallo-β-lactamase VIM-2, demonstrating the first successful preparation of a Co(II)-substituted VIM-2 analogue. Spectroscopic studies of the half- and fully metal loaded enzymes show that both Zn(II) and Co(II) bind cooperatively, where the major species present, regardless of stoichiometry, are apo- and di-Zn (or di… CONTINUE READING