Biocatalyst development by directed evolution.

  title={Biocatalyst development by directed evolution.},
  author={Meng Wang and Tong Si and Huimin Zhao},
  journal={Bioresource technology},
Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly… CONTINUE READING
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