Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

@article{Micke2006BiobankingOF,
  title={Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens},
  author={P. Micke and Mitsuhiro Ohshima and Simin Tahmasebpoor and Z P Ren and Arne {\"O}stman and Fredrik Ponten and J. Botling},
  journal={Laboratory Investigation},
  year={2006},
  volume={86},
  pages={202-211}
}
Molecular tools for tissue profiling, such as expression microarrays and real-time PCR, generally require collection of fresh frozen tissues as sources of high-quality RNA. The fragile nature of RNA prompted us to examine the effects of storage time and transport conditions with regard to RNA integrity and gene expression in nonfixed surgical human specimens. At surgery, fresh normal tonsil and colon tissue was cut into pieces and snap frozen. Additional fresh tissue pieces were (i) left at… CONTINUE READING

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