Bioassay‐guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity

  title={Bioassay‐guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity},
  author={Rosalie Awad and Asim Muhammad and Tony Durst and Vance L. Trudeau and John T. Arnason},
  journal={Phytotherapy Research},
A novel pharmacological mechanism of action for the anxiolytic botanical Melissa officinalis L. (lemon balm) is reported. The methanol extract was identified as a potent in vitro inhibitor of rat brain GABA transaminase (GABA‐T), an enzyme target in the therapy of anxiety, epilepsy and related neurological disorders. Bioassay‐guided fractionation led to the identification and isolation of rosmarinic acid (RA) and the triterpenoids, ursolic acid (UA) and oleanolic acid (OA) as active principles… 
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