Bioanalysis for biocatalysis: multiplexed capillary electrophoresis-mass spectrometry assay for aminotransferase substrate discovery and specificity profiling.

@article{Mironov2013BioanalysisFB,
  title={Bioanalysis for biocatalysis: multiplexed capillary electrophoresis-mass spectrometry assay for aminotransferase substrate discovery and specificity profiling.},
  author={Gleb G. Mironov and Antony D St-Jacques and Alexander Mungham and Matthew G Eason and Roberto A Chica and Maxim V Berezovski},
  journal={Journal of the American Chemical Society},
  year={2013},
  volume={135 37},
  pages={13728-36}
}
In this work, we introduce an entirely automated enzyme assay based on capillary electrophoresis coupled to electrospray ionization mass spectrometry termed MINISEP-MS for multiple interfluent nanoinjections-incubation-separation-enzyme profiling using mass spectrometry. MINISEP-MS requires only nanoliters of reagent solutions and uses the separation capillary as a microreactor, allowing multiple substrates to be assayed simultaneously. The method can be used to rapidly profile the substrate… CONTINUE READING