Bioactive Contaminants Leach from Disposable Laboratory Plasticware

  title={Bioactive Contaminants Leach from Disposable Laboratory Plasticware},
  author={G. Reid McDonald and Alan L. Hudson and Susan M. J. Dunn and Haitao You and Glen B. Baker and Randy M. Whittal and Jonathan W. Martin and Amitabh Jha and Dale E. Edmondson and Andrew Holt},
  pages={917 - 917}
Disposable plasticware such as test tubes, pipette tips, and multiwell assay or culture plates are used routinely in most biological research laboratories. Manufacturing of plastics requires the inclusion of numerous chemicals to enhance stability, durability, and performance. Some lubricating (slip) agents, exemplified by oleamide, also occur endogenously in humans and are biologically active, and cationic biocides are included to prevent bacterial colonization of the plastic surface. We… 
On the disruption of biochemical and biological assays by chemicals leaching from disposable laboratory plasticware.
The nature and sources of leachates are discussed and several examples of compromised bioassay data that speak to the probable widespread nature of this largely unrecognised source of error are reviewed.
Labware Additives Identified to Be Selective Monoamine Oxidase-B Inhibitors
The identification of biologically active substances released from a commonly used plastic microplate were identified by gas chromatography–mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.
Extraction, Identification, and Functional Characterization of a Bioactive Substance From Automated Compound-Handling Plastic Tips
Results demonstrate that solvent-extractable contaminants from some plastic labware used in the contemporary pharmaceutical research and development (R&D) environment can be introduced into physical and biological assays during routine compound management liquid-handling processes.
In plastico : laboratory material Daphnia magna
  • Marek
  • Materials Science
  • 2017
The phenomena of leaching plasticizers1, bioaccumulation of undesirable ingredients from plastic-resin2 and even presence and mobility of potentially toxic compounds in plastics used in surgical
The influence of UV absorbing substances released from plastic containers (leachables) on photometric analyses
Plastic consumables, which are routinely used in the laboratory, can release substances which may subsequently compromise experiments, so called leachables. This was demonstrated in a number of
Characterization of Extractable Species from Polypropylene Microplates
An extractables study performed with available microplates manufactured with plastic, including plates from SiO2 Medical Products containing a plasma treatment designed to produce an ultra-low protein-binding surface is presented.
Comparison of Eppendorf Tubes ® 5.0 mL to conical 15 mL tubes with a focus on releasable UV-absorbing substances (leachables)
It can be shown that a significant amount of leachables is released from the different 15 mL vessels, and for Eppendorf Tubes (and a glass control vessel) these extinctions are so low that photometric detection methods are not compromised.
Chemicals eluting from disposable plastic syringes and syringe filters alter neurite growth, axogenesis and the microtubule cytoskeleton in cultured hippocampal neurons
This is the first study to show that leachates from laboratory consumables can alter the growth of cultured hippocampal neurons, and important considerations are highlighted to ensure leachate contamination does not compromise cell biology experiments.
Oleamide, a Bioactive Compound, Unwittingly Introduced into the Human Body through Some Plastic Food/Beverages and Medicine Containers
Investigation of the migration of oleamide from various plastic, marketed containers for food/beverages and medicines into polymer contact liquid found values are considered of no concern, unless future toxicological studies prove the opposite.
A Review of Plastics Use in Winemaking: HACCP Considerations
Use of plastics is ubiquitous in the food and beverage industries, with expanding usage in wine production. Common plastic additives, used to modify and improve applicability and durability of


Migration studies on fatty acid amide slip additives from plastics into food simulants.
Specific migration studies, using food simulants, have been conducted on a range of polymers which are used in food packaging and contain commonly used fatty acid amide slip additives. Migration
Oleamide: a fatty acid amide signaling molecule in the cardiovascular system?
A physiological role for oleamide in the heart and circulation has yet to be demonstrated, as has production by cells of the cardiovascular system, but this molecule has a range of actions that could give it considerable modulatory power.
Contamination of bottled waters with antimony leaching from polyethylene terephthalate (PET) increases upon storage.
Elevated concentrations of Sb in bottled waters are due mainly to the Sb2O3 used as the catalyst in the manufacture of polyethylene terephthalate (PET(E).