Bioactive Contaminants Leach from Disposable Laboratory Plasticware

@article{McDonald2008BioactiveCL,
  title={Bioactive Contaminants Leach from Disposable Laboratory Plasticware},
  author={G. Reid McDonald and Alan L. Hudson and Susan M. J. Dunn and Haitao You and Glen B. Baker and Randy M. Whittal and Jonathan W. Martin and Amitabh Jha and Dale E. Edmondson and Andrew Holt},
  journal={Science},
  year={2008},
  volume={322},
  pages={917 - 917}
}
Disposable plasticware such as test tubes, pipette tips, and multiwell assay or culture plates are used routinely in most biological research laboratories. Manufacturing of plastics requires the inclusion of numerous chemicals to enhance stability, durability, and performance. Some lubricating (slip) agents, exemplified by oleamide, also occur endogenously in humans and are biologically active, and cationic biocides are included to prevent bacterial colonization of the plastic surface. We… 

On the disruption of biochemical and biological assays by chemicals leaching from disposable laboratory plasticware.

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In plastico : laboratory material Daphnia magna

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The results show COP microcentrifuge tubes perform as well as tubes made of PP, with reduced levels of compounds capable of leaching into solvents typically used in the laboratory.

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It can be shown that a significant amount of leachables is released from the different 15 mL vessels, and for Eppendorf Tubes (and a glass control vessel) these extinctions are so low that photometric detection methods are not compromised.

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Extractables and Leachables in Microcentrifuge Tubes – Extensive HPLC / GC / MS Analysis

Substances leaching out of plastic consumables (leachables) are still underestimated in a majority of life science applications. Increasing scientific evidence shows that this heterogeneous and
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References

SHOWING 1-5 OF 5 REFERENCES

Migration studies on fatty acid amide slip additives from plastics into food simulants.

Specific migration studies, using food simulants, have been conducted on a range of polymers which are used in food packaging and contain commonly used fatty acid amide slip additives. Migration

Contamination of bottled waters with antimony leaching from polyethylene terephthalate (PET) increases upon storage.

Elevated concentrations of Sb in bottled waters are due mainly to the Sb2O3 used as the catalyst in the manufacture of polyethylene terephthalate (PET(E).

Oleamide: a fatty acid amide signaling molecule in the cardiovascular system?

A physiological role for oleamide in the heart and circulation has yet to be demonstrated, as has production by cells of the cardiovascular system, but this molecule has a range of actions that could give it considerable modulatory power.