Bio-Microarray Fabrication Techniques—A Review

  title={Bio-Microarray Fabrication Techniques—A Review},
  author={Irena Barbulovic-Nad and Michael E. Lucente and Yu Sun and Mingjun Zhang and Aaron R. Wheeler and Markus Bussmann},
  journal={Critical Reviews in Biotechnology},
  pages={237 - 259}
ABSTRACT Microarrays with biomolecules (e.g., DNA and proteins), cells, and tissues immobilized on solid substrates are important tools for biological research, including genomics, proteomics, and cell analysis. In this paper, the current state of microarray fabrication is reviewed. According to spot formation techniques, methods are categorized as “contact printing” and “non-contact printing.” Contact printing is a widely used technology, comprising methods such as contact pin printing and… 

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Microfluidic Bioreactors for Cellular Microarrays

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Controlled growth of peptide nanoarrays on Si/SiOx substrates.

A novel route, based on the combination of DPN and ring-opening polymerization of tryptophan-N-carboxyanhydride (Trp-NCA) dendrimer, to generate peptide patterns with varied chain lengths on the nanometer scale is described.

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An equipment-free polydimethylsiloxane microfluidic spotter for fabrication of microarrays.

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A simple and cost-effective procedure for the fabrication of microarrays containing biological samples using a modified bubble-jet printing system is presented, illustrated by the detection of human fragile histidine triad (FHIT), a tumor suppressor gene.

Fast and reliable protein microarray production by a new drop-in-drop technique.

To remarkably increase protein immobilization efficiency on microarray slides the commonly used EDC-NHS system was miniaturized by using a new drop-in-drop printing technique and the very fast UV cross-linking was used to immobilize antibodies.

DNA microarray technology: devices, systems, and applications.

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  • Biology, Chemistry
    Annual review of biomedical engineering
  • 2002
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A Novel PDMS Microfluidic Spotter for Fabrication of Protein Chips and Microarrays

A novel polydimethyl siloxane microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density, and has been applied successfully to proteinmicroarrays for SPR applications.

Microsized 2D protein arrays immobilized by micro-stamps and micro-wells for disease diagnosis and drug screening

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A Highly Parallel Nanoliter Dispenser for Microarray Fabrication

Experiments with oligonucleotides as well as with different proteins showed ascertained carry-over and cross-contamination free printing of DNA and protein microarrays, leading to high quality whilst high throughput microarray fabrication.

Fabricating arrays of single protein molecules on glass using microcontact printing

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