Binding to GDP-agarose identifies a novel 60kDa substrate for the insulin receptor tyrosyl kinase in mouse NIH-3T3 cells expressing high concentrations of the human insulin receptor.


Insulin increased dramatically the tyrosyl phosphorylation of the insulin receptor beta-subunit in mouse NIH-3T3 fibroblasts transfected with the human insulin receptor. Insulin also increased the phosphorylation, on tyrosine residues, of an endogenous 60kDa protein. This protein was identified after being eluted from a GDP-agarose support by GDP. It is… (More)


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