Binding site conformation dictates the color of the dye stains-all. A study of the binding of this dye to the eye lens proteins crystallins.

@article{Sharma1989BindingSC,
  title={Binding site conformation dictates the color of the dye stains-all. A study of the binding of this dye to the eye lens proteins crystallins.},
  author={Yogendra Sharma and Ch Mohan Rao and S. Chenchal Rao and Athul Gopala Krishna and Thayumanasamy Somasundaram and Dorairajan Balasubramanian},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 35},
  pages={20923-7}
}
The interaction of the cationic carbocyanine dye Stains-all (1-ethyl-2-[3-(1-ethyl-naphthol[1,2-d]thiazolin-2-ylidene)-2- methylpropenyl]naphthol[1,2-d]thiazolium bromide) with the eye lens proteins crystallins has been studied. alpha- and gamma-crystallins do not bind the dye, while beta- and delta-crystallins do, consistent with the fact that the latter two proteins bind the calcium ion. beta-Crystallin resembles parvalbumin in that it induces only the J-band of the bound dye. delta… CONTINUE READING