Binding-protein-dependent arginine transport in Pasteurella haemolytica.

  title={Binding-protein-dependent arginine transport in Pasteurella haemolytica.},
  author={Laura S. Caskey and James G. Lamphear and Sarah K. Highlander},
  volume={142 ( Pt 7)},
A periplasmic arginine transport system that is a member of the ATP -dependent transport superfamily was identified in Pasteurella haemolytica. The gene encoding the periplasmic binding protein (lapT) was cloned and the protein overexpressed in Escherichia coli. LapT was purified to homogeneity using a modified osmotic shock procedure and anion-exchange… CONTINUE READING