Binding properties of a monoclonal antibody directed toward lead-chelate complexes.

@article{Khosraviani2000BindingPO,
  title={Binding properties of a monoclonal antibody directed toward lead-chelate complexes.},
  author={Mehraban Khosraviani and Robert C. Blake and Andrey R. Pavlov and Stanley C. Lorbach and H Yu and James B. Delehanty and Martin W Brechbiel and Diane A. Blake},
  journal={Bioconjugate chemistry},
  year={2000},
  volume={11 2},
  pages={
          267-77
        }
}
A monoclonal antibody (2C12) that recognizes a Pb(II)-cyclohexyldiethylenetriamine pentaacetic acid complex was produced by the injection of BALB/c mice with a Pb(II)-chelate complex covalently coupled to a carrier protein. The ability of purified antibody to interact with a variety of metal-free chelators and metal-chelate complexes was assessed by measuring equilibrium dissociation constants. The antibody bound to metal-free trans-cyclohexyldiethylenetriamine pentaacetic acid (CHXDTPA) with… 
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A monoclonal antibody that recognizes cadmium-EDTA complexes has been produced by the injection of BALB/c mice with a metal-chelate complex covalently coupled to a carrier protein. The ability of
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TLDR
Competitive metal binding immunoassays revealed an unusual behavior by Zn(II) which remains to be explained, and IMAC allowed us to predict the metal binding amino acids involved in the antibody paratope, and to guide the screening of its catalytic potential.
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TLDR
These anti-metal chelate MAbs have potential use as vehicles for the pretargeted delivery of radiometal chelates to tumors.
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TLDR
While none of the DTPA-derivative chelates could challenge DOTA in its ability to hold the radioytrium, significant differences were observed in the kinetic inertness of the A and B isomers of CHX, indicating that the CHX-B ligand is not as suitable for 90Y-labeling of Mabs.
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