Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin.

@article{Lui2003BindingPF,
  title={Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin.},
  author={Winnie W.Y. Lui and Brett M Collins and Jennifer Hirst and Alison M. Motley and Caroline Millar and Peter Schu and David R. Owen and Margaret S Robinson},
  journal={Molecular biology of the cell},
  year={2003},
  volume={14 6},
  pages={
          2385-98
        }
}
The adaptor appendage domains are believed to act as binding platforms for coated vesicle accessory proteins. Using glutathione S-transferase pulldowns from pig brain cytosol, we find three proteins that can bind to the appendage domains of both the AP-1 gamma subunit and the GGAs: gamma-synergin and two novel proteins, p56 and p200. p56 elicited better antibodies than p200 and was generally more tractable. Although p56 and gamma-synergin bind to both GGA and gamma appendages in vitro… CONTINUE READING

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