Binding of the Tn3 transposase to the inverted repeats of Tn3.

@article{New1988BindingOT,
  title={Binding of the Tn3 transposase to the inverted repeats of Tn3.},
  author={J H New and Angela K. Eggleston and Michael Fennewald},
  journal={Journal of molecular biology},
  year={1988},
  volume={201 3},
  pages={589-99}
}
The transposase protein and the inverted repeat sequences of Tn3 are both essential for Tn3 cointegrate formation and transposition. We have developed two assays to detect site-specific binding of transposase to the inverted repeats: (1) a nitrocellulose filter binding assay in which transposase preferentially retains DNA fragments containing inverted repeat sequences, and (2) a DNase 1 protection assay in which transposase prevents digestion of the inverted repeats by DNase 1. Both assays show… CONTINUE READING