Binding of the O-antigen of Shigella dysenteriae type 1 and 26 related synthetic fragments to a monoclonal IgM antibody.

  title={Binding of the O-antigen of Shigella dysenteriae type 1 and 26 related synthetic fragments to a monoclonal IgM antibody.},
  author={Viliam Pavliak and Eugenia M. Nashed and Vince Pozsgay and Pavol Kov{\'a}{\vc} and Arthur B. Karpas and Chiayung Chu and Rachel Schneerson and John B. Robbins and Cornelis P.J. Glaudemans},
  journal={The Journal of biological chemistry},
  volume={268 34},
Shigella dysenteriae type 1 possesses an O-antigen whose repeating unit is -->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->2)-alpha-D-Galp -(1-->3)-alpha-D- GlcpNAc-(1-->, where Rhap is rhamnopyranosyl, Galp is galactopyranosyl, and Glcp is glucopyranosyl. Using ligand-induced protein fluorescence change, we have measured the affinities of a monoclonal murine IgM for 26 fragments of, or related to, the structure of the O-polysaccharide and of the IgM Fab for the intact O-specific bacterial… Expand
Conformation of the O-specific polysaccharide of Shigella dysenteriae type 1: molecular modeling shows a helical structure with efficient exposure of the antigenic determinant alpha-L-Rhap-(1-->2)-alpha-D-Galp.
The conformational properties of fragments of this O-antigen were explored using systematic search with a modified HSEA method (GLYCAN) and with molecular mechanics MM3(96), which indicates that the trisaccharide B-C-D and tetrasaccharides containing the B-D moiety exist as two different conformers, distinguished by the conformations I and II of the C-D linkage. Expand
Of four murine, anti-Shigella dysenteriae type 1 O-polysaccharide antibodies, three employ V-genes that differ extensively from those of the fourth.
Three murine, monoclonal antibodies were generated against Shigella dysenteriae type 1 O-specific polysaccharide (O-SP)-conjugate and showed 93% homology, but were quite different to the primary sequence of IgM 3707 E9, of the same O-SP-specificity, previously reported. Expand
Conformation of the branched O-specific polysaccharide of Shigella dysenteriae type 2: molecular mechanics calculations show a compact helical structure exposing an epitope which potentially mimics galabiose.
Conformational analyses of the branched repeating unit of the O-antigenic polysaccharide of Shigella dysenteriae type 2 have been performed with molecular mechanics MM3, and it appears possible that the alpha-D-Glc-(1-->4)-beta- D-Gal moiety can evade recognition by the immune system of the host by the mimicry of globo structures. Expand
Effect of the nonreducing end of Shigella dysenteriae type 1 O-specific oligosaccharides on their immunogenicity as conjugates in mice
The synthetic oligosaccharides corresponding to the tetrasaccharide repeating unit of the O-SP of Shigella dysenteriae type 1 covalently linked to human serum albumin elicited O-sp-specific IgG in mice and inhibited the binding of serum raised against whole bacteria to its LPS to a similar extent but lower than the native O- SP. Expand
Investigation by NMR Spectroscopy and Molecular Modeling of the Conformations of Some Modified Disaccharide Antigens for Shigella Dysenteriae Type 1
Abstract The O-polysaccharide of Shigella dysenteriae type 1 is made up of multiple repeats of the linear tetrasaccharide 3)-α-L-Rhap-(1→2)-α-D-Galp-(1→3)-α-D-GlcpNAc-(1→3)-α-L-Rhap-(1→, for whichExpand
Circular dichroism of the O-specific polysaccharide of Vibrio cholerae O1 and some related derivatives.
The O-specific polysaccharide of Vibrio cholerae O1 is a homopolymer of alpha-(1 --> 2)-linked 4-amino-4, 6-dideoxy-D-mannopyranose whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid, and the sign of the CD was found negative in all solvents used. Expand
Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine1
It was demonstrated that induction of an efficient serotype 2a-specific anti-O-Ag Ab response was dependent on the length of the oligosaccharide sequence, which was a potential candidate for further development of a chemically defined glycoconjugate vaccine against S. flexneri 2a infection. Expand
Immunochemical characterization of O polysaccharides composing the alpha-D-rhamnose backbone of lipopolysaccharide of Pseudomonas syringae and classification of bacteria into serogroups O1 and O2 with monoclonal antibodies
Murine monoclonal antibodies reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra- and tri-alpha-D-rhamnose repeats in the backbone generated and used for immunochemical analysis and for serological classification of the bacteria. Expand
Synthesis of hexa- to tridecasaccharides related to Shigella dysenteriae type 1 for incorporation in experimental vaccines.
Hexa- to tridecasaccharides corresponding to the O-specific polysaccharide of the Gram-negative bacterium Shigella dysenteriae type 1 were synthesized in solution phase and contain the 5-(methoxycarbonyl)pentyl aglycon for eventual attachment to immunogenic carriers using a recently published protocol. Expand
Protein conjugates of synthetic saccharides elicit higher levels of serum IgG lipopolysaccharide antibodies in mice than do those of the O-specific polysaccharide from Shigella dysenteriae type 1.
Serum IgG anti-LPS, elicited in mice by O-SP-HSA or synthetic tetra-, octa-, dodeca-, and hexadecasaccharide fragments, was measured by ELISA and the highest geometric mean levels of IgGAntiLPS were elicited by the hexadecamer with 9 chains or 9 moles of saccharide/HSA and the octamer with 20 chains and the dodecamer with 10 chains. Expand


Synthetic oligosaccharides related to Group B streptococcal polysaccharides. The rhamnotriose moiety of the common antigen
The methyl glycoside (1) of the α-L-Rhap-(1 → 2)-α-L-Rhap-(1 → 2)-α-L-Rhap-(1 → portion of the group-specific, common antigen polysaccharide of Group B Streptococci has been synthesized. The keyExpand
Carbohydrates : synthetic methods and applications in medicinal chemistry
Part 1 Synthetic methods of carbohydrate by chemicals: efficient reaction channels from mono-and disaccharides to enaniopure building blocks and exploitation of their application profiles, F.W.Expand
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