Binding of the N-terminal 63 kDa portion of connectin/titin to alpha-actinin as revealed by the yeast two-hybrid system.

@article{Ohtsuka1997BindingOT,
  title={Binding of the N-terminal 63 kDa portion of connectin/titin to alpha-actinin as revealed by the yeast two-hybrid system.},
  author={H Ohtsuka and Haruaki Yajima and Katsuhiko Maruyama and Satoshi Kensuke Kimura},
  journal={FEBS letters},
  year={1997},
  volume={401 1},
  pages={65-7}
}
Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones coding the C-terminal half region of alpha-actinin (amino acids, 343-897 and 446-897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of alpha-actinin and the N… CONTINUE READING

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