Binding of recA protein to Z-form DNA studied with circular and linear dichroism spectroscopy.

@article{Takahashi1989BindingOR,
  title={Binding of recA protein to Z-form DNA studied with circular and linear dichroism spectroscopy.},
  author={Masayuki Takahashi and Mikael Kubista and B. Nelson Norden},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 15},
  pages={8568-74}
}
Binding of RecA to poly(dG-m5dC) and poly(dG-dC) under B- and Z-form conditions was studied using circular dichroism (CD) and linear dichroism (LD). LD revealed a quantitative binding of RecA to Mg2+-induced Z-form poly(dG-m5dC) with a stoichiometry of 3.1 base pairs/RecA monomer, which is slightly larger than the 2.7 base pairs observed for the B-form. The… CONTINUE READING