Binding of leukotrienes C4 and D4 to membranes from guinea pig lung: regulation by ions and guanine nucleotides.


Tritium-labeled leukotrienes C4 and D4 (LTC4 and LTD4) bind to membranes from guinea pig lung. Binding properties of the two ligands are almost identical. More than 80% of 3H-LTC4 and 3H-LTD4 binding can be blocked by unlabeled LTC4 (IC50 8 nM versus 3H-LTC4 and 8 nM versus 3H-LTD4), LTD4 (12 nM, 16 nM), LTE4 (40 nM, 98 nM), and the leukotriene antagonist… (More)