Binding of hemoglobin by Porphyromonas gingivalis.

  title={Binding of hemoglobin by Porphyromonas gingivalis.},
  author={Atsuo Amano and Masae Kuboniwa and Kosuke Kataoka and Kazuko Tazaki and Eiji Inoshita and Hideki Nagata and Hiroo Tamagawa and Satoshi Shizukuishi},
  journal={FEMS microbiology letters},
  volume={134 1},
In this study, we investigated whether Porphyromonas gingivalis can bind hemoglobin as an initial step in the acquisition of heme from hemoglobin. The binding of human hemoglobin by P. gingivalis cells was determined using [3H]hemoglobin. Hemoglobin binding occurred rapidly, reversibly and specifically. A Scatchard analysis of the binding data generated a linear plot, indicating a single population of binding proteins. The apparent Kd was 1.0 +/- 0.19 x 10(-6) M and there were 3.2 +/- 0.76 x 10… CONTINUE READING

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