Binding of calcium and phosphate ions to dentin phosphophoryn.

@article{Marsh1989BindingOC,
  title={Binding of calcium and phosphate ions to dentin phosphophoryn.},
  author={Mary E. Marsh},
  journal={Biochemistry},
  year={1989},
  volume={28 1},
  pages={346-52}
}
The concomitant binding of calcium and inorganic phosphate ions by the highly phosphorylated rat dentin phosphophoryn (HP) was measured in the pH range of 7.4-8.5 using an ultrafiltration procedure. HP binds almost exclusively the triply charged PO4(3-)ion, and for each PO4(3-)ion bound, the protein binds about 1.5 additional Ca2+ ions. The protein-mineral ion complex can be described as a protein with two different ligands, Ca2+ ions and calcium phosphate clusters having a stoichiometry of… CONTINUE READING