Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage.

@article{Glander1999BindingOA,
  title={Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage.},
  author={H. J. Glander and Jana Schaller},
  journal={Molecular human reproduction},
  year={1999},
  volume={5 2},
  pages={109-15}
}
When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing… CONTINUE READING
69 Citations
19 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 69 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 19 references

A new combined in-vitro test model for the identification of substances affecting essential sperm functions.Hum. Reprod

  • W ̈gele
  • 1997

Optimisation of cryopreservation of subfertile sperm samples. A computer-assisted study

  • U. Paasch, Glander, H.-J
  • 1997

Evaluation of a computeraided semen analysis system with sperm tail detection

  • J. G. de Wijchman, B. T. Wolf, S. Jager
  • 1995

The complexity of the phospholipid binding protein annexin V

  • W. L. Van Heerde, P. G. Degroot, C.P.M. Reutelingsperger
  • 1995
1 Excerpt

Similar Papers

Loading similar papers…