Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor.

@article{Jenness1983BindingOA,
  title={Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor.},
  author={D. Jenness and Anne C. Burkholder and Leland H. Hartwell},
  journal={Cell},
  year={1983},
  volume={35 2 Pt 1},
  pages={
          521-9
        }
}
The division cycle of yeast a cells is inhibited by alpha-factor. Haploid a cells were found to bind 35S-labeled alpha-factor, whereas haploid alpha cells and diploid a/alpha cells showed little binding. The association of alpha-factor with a cells was reversible upon dilution. Unlabeled alpha-factor competed for binding of 35S-alpha-factor; the concentration dependence for competition indicated 9 X 10(5) binding sites per cell with a dissociation constant (KD) of 3 X 10(-7) M. The rates of… CONTINUE READING

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