Binding of XPA and RPA to damaged DNA investigated by fluorescence anisotropy.

@article{Hey2001BindingOX,
  title={Binding of XPA and RPA to damaged DNA investigated by fluorescence anisotropy.},
  author={Thomas Hey and Georg Lipps and Gerhard Krauss},
  journal={Biochemistry},
  year={2001},
  volume={40 9},
  pages={2901-10}
}
The proteins XPA and RPA are assumed to be involved in primary damage recognition of global genome nucleotide excision repair. XPA as well as RPA have been each reported to specifically bind DNA lesions, and ternary complex formation with damaged DNA has also been shown. We employed fluorescence anisotropy measurements to study the DNA-binding properties of XPA and RPA under true equilibrium conditions using damaged DNA probes carrying a terminal fluorescein modification as a reporter. XPA… CONTINUE READING
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