Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks.

@article{Blier1993BindingOK,
  title={Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks.},
  author={Peter R. Blier and Andrew J. Griffith and Joseph F Craft and John A. Hardin},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 10},
  pages={7594-601}
}
Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded DNA (dsDNA) and can bind at multiple sites along dsDNA in an energy-independent manner. Its function in vivo is unknown, but it has been implicated in both DNA replication and repair and in transcriptional control. We have used an electrophoretic mobility shift assay to further define the DNA binding properties of the Ku protein. Titration… CONTINUE READING

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