Binding of Actinobacillus pleuropneumoniae Lipopolysaccharides to Glycosphingolipids Evaluated by Thin-Layer Chromatography

@article{AbulMilh1999BindingOA,
  title={Binding of Actinobacillus pleuropneumoniae Lipopolysaccharides to Glycosphingolipids Evaluated by Thin-Layer Chromatography},
  author={M. Abul-Milh and S. Paradis and J. Dubreuil and M. Jacques},
  journal={Infection and Immunity},
  year={1999},
  volume={67},
  pages={4983 - 4987}
}
ABSTRACT The binding profile of Actinobacillus pleuropneumoniaeserotypes 1 and 2 to various glycosphingolipids was evaluated by using thin-layer chromatogram overlay. A. pleuropneumoniae whole cells recognized glucosylceramide (Glcβ1Cer), galactosylceramide (Galβ1Cer) with hydroxy and nonhydroxy fatty acids, sulfatide (SO3-3Galβ1Cer), lactosylceramide (Galβ1-4Glcβ1Cer), gangliotriaosylceramide GgO3(GalNAcβ1-4Galβ1-4Glcβ1Cer), and gangliotetraosylceramide GgO4 (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1Cer… Expand
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