Binding of 14C-furosemide to isolated human erythrocytes.

@article{Paci1989BindingO1,
  title={Binding of 14C-furosemide to isolated human erythrocytes.},
  author={Angelo Paci and Franca Cocci and Giuliano Ciarimboli and Franca Piras and Andrew Tonelli and Sergio Ghione},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={1989},
  volume={7 12},
  pages={1459-65}
}
The incubation of 14C-furosemide at high specific activity with intact red blood cells at 37 degrees C, pH 7.4, has enabled the furosemide binding sites to be characterized with respect to time course, affinity and specificity. The binding reaction was rapid, reversible and close to thermodynamic equilibrium. Binding was dependent on cell and furosemide concentration and was saturable. At equilibrium, pharmacological doses of furosemide competitively inhibited 14C-furosemide binding with 50… CONTINUE READING

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