Binding of [3H]DMCM, a convulsive benzodiazepine ligand, to rat brain membranes: preliminary studies.

@article{Braestrup1983BindingO,
  title={Binding of [3H]DMCM, a convulsive benzodiazepine ligand, to rat brain membranes: preliminary studies.},
  author={Claus Braestrup and Mogens Brondsted Nielsen and Tage Honor{\'e}},
  journal={Journal of neurochemistry},
  year={1983},
  volume={41 2},
  pages={454-65}
}
DMCM (methyl 6,7-dimethoxy-4-ethyl-beta-carboline-3-carboxylate) produces convulsions in mice and rats, probably by interacting with benzodiazepine (BZ) receptors. Investigation of specific binding of [3H]DMCM to rat hippocampus and cortex revealed polyphasic saturation curves, indicating a high-affinity site (KD = 0.5-0.8 nM) and a site with lower affinity (KD = 3-6 nM). BZ receptor ligands of various chemical classes, but not other agents, displace [3H]DMCM from specific binding sites… CONTINUE READING