Binding measurements of indolocarbazole derivatives to immobilised human serum albumin by high-performance liquid chromatography.

@article{Ashton1996BindingMO,
  title={Binding measurements of indolocarbazole derivatives to immobilised human serum albumin by high-performance liquid chromatography.},
  author={D. S. Ashton and Christopher R. Beddell and G Stuart Cockerill and Kishorchandra Gohil and C Gowrie and James E Robinson and Martin J Slater and Kl{\'a}ra L Valk{\'o}},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1996},
  volume={677 1},
  pages={194-8}
}
The binding properties of six indolocarbazole derivative have been measured using immobilised human serum albumin (HSA) in an HPLC column. The compounds showed very strong binding to HSA which necessitated the application of a 30 to 40% concentration of 2-propanol in the mobile phase. This represents a much higher concentration than is recommended by the column manufacturers. This HSA column had not changed its binding property when it was used again with 4% 2-propanol and 96% phosphate buffer… CONTINUE READING

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