Binding assay for thyrotropin receptor autoantibodies using the recombinant receptor protein.

@article{Costagliola1992BindingAF,
  title={Binding assay for thyrotropin receptor autoantibodies using the recombinant receptor protein.},
  author={Sabine Costagliola and St{\'e}phane Swillens and P Niccoli and Jacques E. Dumont and G. Vassart and Marian Ludgate},
  journal={The Journal of clinical endocrinology and metabolism},
  year={1992},
  volume={75 6},
  pages={1540-4}
}
We have characterized a transfected Chinese hamster ovary cell line, JP09, which expresses high levels of the human TSH receptor (TSH-R). Based on a theoretical biological activity for TSH of 40 IU/mg, JP09 has approximately 90,000 receptors per cell, having a dissociation constant of 1.64 x 10(3) mU/L or 1.47 x 10(-9) mol/L. We have used JP09 to prepare solubilized TSH-Rs which have formed the basis of a binding assay for thyroid-binding inhibiting immunoglobulins in unfractionated sera. We… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

Similar Papers

Loading similar papers…