Binding assay for incorporation of alkali-extractable proteins in the Saccharomyces cerevisiae cell wall.

@article{Tepari2007BindingAF,
  title={Binding assay for incorporation of alkali-extractable proteins in the Saccharomyces cerevisiae cell wall.},
  author={Renata Tepari{\'c} and Igor Stuparevi{\'c} and Vladimir Mr{\vs}a},
  journal={Yeast},
  year={2007},
  volume={24 4},
  pages={259-66}
}
Yeasts have developed three different ways of attaching proteins to cell wall glucan. Some proteins are bound to beta-1,3-glucan non-covalently, while others are attached covalently, through GPI-anchor and beta-1,6-glucan, or directly to beta-1,3-glucan by alkali-labile ester linkage between the gamma-carboxyl groups of glutamic acid and the hydroxyl groups of glucoses (Pir proteins). In order to obtain further insight into the binding mechanism, a novel, simple binding assay for Pir-family… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.