Binding and hydrodynamic properties of muscarinic receptor subtypes solubilized in 3-(3-cholamidopropyl)dimethylammonio-2-hydroxy-1-propanesulfonate.

@article{Poyner1989BindingAH,
  title={Binding and hydrodynamic properties of muscarinic receptor subtypes solubilized in 3-(3-cholamidopropyl)dimethylammonio-2-hydroxy-1-propanesulfonate.},
  author={David R Poyner and Nigel J. M. Birdsall and Christine A Curtis and P C Eveleigh and Edward C. Hulme and E K Pedder and Mark Wheatley},
  journal={Molecular pharmacology},
  year={1989},
  volume={36 3},
  pages={420-9}
}
The muscarinic receptor from the cerebral cortex, heart, and lacrimal gland can be solubilized in the zwitterionic detergent 3-(3-cholamidopropyl)dimethylammonio-2-hydroxy-1-propane sulfonate (CHAPSO) with retention of high affinity [3H]N-methyls-copolamine binding. However, in this detergent there are significant differences in the binding properties of the receptors, compared with those observed in membranes and digitonin solution. Some agents retain a degree of selectivity. In the heart and… CONTINUE READING