Binding and Clearance of Radioactive Adrenaline and Noradrenaline in Sheep Blood

@article{ElBahr2006BindingAC,
  title={Binding and Clearance of Radioactive Adrenaline and Noradrenaline in Sheep Blood},
  author={Sabry Mohamed El-Bahr and Hermann Kahlbacher and Martina Patzl and Rupert Palme},
  journal={Veterinary Research Communications},
  year={2006},
  volume={30},
  pages={423-432}
}
An understanding of the conditions influencing protein binding of catecholamines (CAs) is important in studying their metabolic effects. Unfortunately, reports on plasma protein binding of CAs are scarce, conflicting and mainly performed in vitro.The aim of our in vivo and in vitro studies was to investigate binding and clearance of radioactive adrenaline (epinephrine) (3H-A), noradrenaline (norepinephrine) (3H-NA) and their metabolites in sheep blood. The time course of the radioactivity in… 
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References

SHOWING 1-10 OF 16 REFERENCES
The physiological disposition of H3-epinephrine and its metabolite metanephrine.
TLDR
The disappearance of H 3 -epinephrine from plasma after an intravenous injection has two phases: an initial rapid fall, reflecting diffusion into tissues and O-methylation, followed by a slower decline indicating a gradual release from binding sites and concurrent metabolism.
Catecholamine Binding Protein: Binding of Tritium to a Specific Protein Fraction of Human Plasma following in vitro Incubation with Tritiated Noradrenaline
TLDR
The present investigation was undertaken to determine whether circulating catecholamines were bound to a specific protein component of plasma (catecholamine binding protein) in a manner analogous to corticosteroid binding globulin9 or thyroxine binding Globulin10.
The binding of catecholamines to human serum proteins.
  • G. Powis
  • Biology, Medicine
    Biochemical pharmacology
  • 1975
Binding of adrenaline and isoprenaline to plasma proteins of the dog.
TLDR
It is concluded that the N substitution present in adrenaline and isoprenaline appears to be responsible for the different degrees of protein binding.
Not norepinephrine but its oxidation products bind specifically to plasma proteins.
TLDR
Binding studies of tritiated norepinephrine to human serum albumin found specific binding to be an artifact, due to the presence of one or more oxidation products of NE which are rapidly formed at pH greater than 6.5, and the necessity for careful investigation of the purity and stability of the labeled ligand.
Binding of catecholamines to human serum albumin.
  • A. Danon, J. Sapira
  • Biology, Chemistry
    The Journal of pharmacology and experimental therapeutics
  • 1972
TLDR
The binding of 14C-labeled norepinephrine and epinephrine to human serum albumin was studied at pH 7.4 and room temperature and the extremely low binding capacity suggested that the active species might not be albumin but rather a second protein.
Plasma protein binding of catecholamines, prazosin and propranolol in diabetes mellitus
TLDR
The results suggest that non-enzymatic glycosylation of plasma proteins may increase the unbound fraction of the adrenergic blockers prazosin and propranolol.
A dopamine transporter in human erythrocytes: modulation by insulin
TLDR
It is shown that catecholamines, and in particular dopamine, are transported in red blood cells via an exchanger which is possibly the choline transport system, and the activity of this transporter is regulated by insulin.
In vitro binding study of epinephrine and bovine serum albumin.
TLDR
It appeared that there was a fast exchange rate between bound and unbound epinephrine and that the most probable active site on the small molecule was the alkyl side chain.
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