Binding Interaction of the Heregulinβ egf Domain with ErbB3 and ErbB4 Receptors Assessed by Alanine Scanning Mutagenesis*

@article{Jones1998BindingIO,
  title={Binding Interaction of the Heregulin$\beta$ egf Domain with ErbB3 and ErbB4 Receptors Assessed by Alanine Scanning Mutagenesis*},
  author={Jennifer T. Jones and Marcus D. Ballinger and Paul I. Pisacane and Julie A. Lofgren and V. Danial Fitzpatrick and Wayne J. Fairbrother and James A. Wells and Mark X. Sliwkowski},
  journal={The Journal of Biological Chemistry},
  year={1998},
  volume={273},
  pages={11667 - 11674}
}
Individual residues of the heregulinβ (HRG)egf domain were mutated to alanine and displayed monovalently on phagemid particles as gene III fusion proteins. Wild type HRGβ egf domain displayed on phage was properly folded as evidenced by its ability to bind ErbB3 and ErbB4 receptor-IgG fusion proteins with affinities close to those measured for bacterially produced HRGβ egf domain. Binding to ErbB3 and ErbB4 receptors was affected by mutation of residues throughout theegf domain; including the… 

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