Binding Epitope of Somatostatin Defined by Phage-Displayed Peptide Libraries

@article{Wright1995BindingEO,
  title={Binding Epitope of Somatostatin Defined by Phage-Displayed Peptide Libraries},
  author={Richard M. Wright and Hermann Gram and Anthony Vattay and Susan Byrne and Phil Lake and Diane Dottavio},
  journal={Bio/Technology},
  year={1995},
  volume={13},
  pages={165-169}
}
We have developed a versatile phagemid system to display peptides on the surface of M13 bacteriophage at a copy number which approaches monovalency. In this system, a phagemid encodes a peptide fused to the ammo-terminus of the second domain (dII) of the minor coat protein pIII under control of the inducible lac promoter. The fusion protein is displayed in combination with several copies of wild-type pIII on the surface of phage. Two diverse random octapeptide libraries, one linear and one… CONTINUE READING

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