Bimolecular fluorescence complementation: lighting up seven transmembrane domain receptor signalling networks.

@article{Rose2010BimolecularFC,
  title={Bimolecular fluorescence complementation: lighting up seven transmembrane domain receptor signalling networks.},
  author={Rachel H Rose and Stephen J Briddon and Nicholas D. Holliday},
  journal={British journal of pharmacology},
  year={2010},
  volume={159 4},
  pages={738-50}
}
There is increasing complexity in the organization of seven transmembrane domain (7TM) receptor signalling pathways, and in the ability of their ligands to modulate and direct this signalling. Underlying these events is a network of protein interactions between the 7TM receptors themselves and associated effectors, such as G proteins and beta-arrestins. Bimolecular fluorescence complementation, or BiFC, is a technique capable of detecting these protein-protein events essential for 7TM receptor… CONTINUE READING