Bimodal lipid substrate dependence of phosphatidylinositol kinase.

Abstract

Phosphatidylinositol (PI) kinase activity was solubilized from rat liver microsomes and partially purified by chromatography on hydroxyapatite and Reactive Green 19-Superose. Examination of the ATP dependence using a mixed micellar assay gave a Km of 120 microM. The dependence of reaction rate on PI was more complicated. PI kinase bound a large amount of… (More)

Topics

Cite this paper

@article{Ganong1990BimodalLS, title={Bimodal lipid substrate dependence of phosphatidylinositol kinase.}, author={Barry R. Ganong}, journal={Biochemistry}, year={1990}, volume={29 29}, pages={6904-10} }