Bimane Fluorescence Scanning Suggests Secondary Structure near the S3-S4 Linker of BK Channels*

@article{Semenova2009BimaneFS,
  title={Bimane Fluorescence Scanning Suggests Secondary Structure near the S3-S4 Linker of BK Channels*},
  author={N. Semenova and K. Abarca-Heidemann and Eva Loranc and B. S. Rothberg},
  journal={Journal of Biological Chemistry},
  year={2009},
  volume={284},
  pages={10684 - 10693}
}
  • N. Semenova, K. Abarca-Heidemann, +1 author B. S. Rothberg
  • Published 2009
  • Chemistry, Medicine
  • Journal of Biological Chemistry
    • Gating of large conductance Ca2+-activated K+ channels (BK or maxi-K channels) is controlled by a Ca2+-sensor, formed by the channel cytoplasmic C-terminal domain, and a voltage sensor, formed by its S0-S4 transmembrane helices. Here we analyze structural properties of a portion of the BK channel voltage sensing domain, the S3-S4 linker, using fluorescence lifetime spectroscopy. Single residues in the S3-S4 linker region were substituted with cysteine, and the cysteine-substituted mutants were… CONTINUE READING
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