Bimane Fluorescence Scanning Suggests Secondary Structure near the S3-S4 Linker of BK Channels*

@article{Semenova2009BimaneFS,
  title={Bimane Fluorescence Scanning Suggests Secondary Structure near the S3-S4 Linker of BK Channels*},
  author={N. Semenova and K. Abarca-Heidemann and Eva Loranc and B. S. Rothberg},
  journal={Journal of Biological Chemistry},
  year={2009},
  volume={284},
  pages={10684 - 10693}
}
  • N. Semenova, K. Abarca-Heidemann, +1 author B. S. Rothberg
  • Published 2009
  • Chemistry, Medicine
  • Journal of Biological Chemistry
  • Gating of large conductance Ca2+-activated K+ channels (BK or maxi-K channels) is controlled by a Ca2+-sensor, formed by the channel cytoplasmic C-terminal domain, and a voltage sensor, formed by its S0-S4 transmembrane helices. Here we analyze structural properties of a portion of the BK channel voltage sensing domain, the S3-S4 linker, using fluorescence lifetime spectroscopy. Single residues in the S3-S4 linker region were substituted with cysteine, and the cysteine-substituted mutants were… CONTINUE READING
    26 Citations
    Relative transmembrane segment rearrangements during BK channel activation resolved by structurally assigned fluorophore–quencher pairing
    • 30
    • PDF
    Identification of a pre-active conformation of a pentameric channel receptor
    • 20
    • PDF
    The trajectory of discrete gating charges in a voltage-gated potassium channel
    • PDF
    β1-subunit–induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel
    • 11
    • PDF
    K(+) channels: function-structural overview.
    • 111
    • PDF

    References

    SHOWING 1-10 OF 53 REFERENCES
    A Role for the S0 Transmembrane Segment in Voltage-dependent Gating of BK Channels
    • 49
    • PDF
    Short-range Molecular Rearrangements in Ion Channels Detected by Tryptophan Quenching of Bimane Fluorescence
    • 40
    • PDF
    Role of Charged Residues in the S1–S4 Voltage Sensor of BK Channels
    • 153
    • PDF
    Position and Role of the BK Channel α Subunit S0 Helix Inferred from Disulfide Crosslinking
    • 59
    • PDF