Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications

@article{Durand2012BilirubinOF,
  title={Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications},
  author={Fabien Durand and S{\'e}bastien Gounel and Christian Hauge Kjaergaard and Edward I. Solomon and Nicolas Mano},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={96},
  pages={1489-1498}
}
A novel bilirubin oxidase (BOD), from the rice blast fungus Magnaporthe oryzae, has been identified and isolated. The 64-kDa protein containing four coppers was successfully overexpressed in Pichia pastoris and purified to homogeneity in one step. Protein yield is more than 100 mg for 2 L culture, twice that of Myrothecium verrucaria. The k cat/K m ratio for conjugated bilirubin (1,513 mM−1 s−1) is higher than that obtained for the BOD from M. verrucaria expressed in native fungus (980 mM−1 s−1… CONTINUE READING
9 Citations
61 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 61 references

Nouvelle Bilirubine Oxydase de Bacillus pumilus

  • F Durand, N Mano
  • Centre National de la Recherche Scientifique, Fr…
  • 2010
Highly Influential
4 Excerpts

Bilirubin oxidase from Trachyderma tsunodae K-2593, a multi-copper enzyme

  • K Hiromi, S Yamaguchi, Y Sugiura, H Iwamoto, J Hirose
  • Biosci Biotech Biochem
  • 1992
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…