Bile acid synthesis in man. II. Determination of 7 alpha-hydroxycholesterol, (22R)-22-hydroxycholesterol, and 26-hydroxycholesterol in human meconium.

@article{Lavy1977BileAS,
  title={Bile acid synthesis in man. II. Determination of 7 alpha-hydroxycholesterol, (22R)-22-hydroxycholesterol, and 26-hydroxycholesterol in human meconium.},
  author={U. I. Lavy and Sumner H. Burstein and Marcel Gut and Norman B. Javitt},
  journal={Journal of lipid research},
  year={1977},
  volume={18 2},
  pages={
          232-8
        }
}
7 alpha-Hydroxycholesterol, (22R)-22-hydroxycholesterol and 26-hydroxycholesterol have been quantitated in human meconium. The method used tetrahydrofuran for extraction and solvolysis of the sulfate esters, liquid partition chromatography for the separation of the hydroxysterols, gas-liquid chromatography for quantitation, gas-liquid chromatography-mass spectrometry for identification, and tritiated and 14C-labeled tracers for overall recovery standards. (22R)-22-Hydroxycholesterol and 26… CONTINUE READING