Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT.

@article{But2013BifunctionalSP,
  title={Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT.},
  author={Sergey Y. But and Valentina N. Khmelenina and Alexander S. Reshetnikov and Yuri Aleksandrovich Trotsenko},
  journal={FEMS microbiology letters},
  year={2013},
  volume={347 1},
  pages={43-51}
}
The aerobic obligate methylotroph Methylobacillus flagellatus KT was shown to synthesize sucrose in the presence of 0.5-2% NaCl in the growth medium. In the genome of this bacterium, an open reading frame (ORF) encoding a predicted 84-kD polypeptide homologous to the plant and cyanobacterial sucrose phosphate synthases (SPSs) was found. Using heterologous expression of the putative sps gene in Escherichia coli, followed by affinity chromatography, pure recombinant protein SPS-His6 was obtained… CONTINUE READING
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