Bifunctional role of leucine 300 of firefly luciferase in structural rigidity.

Abstract

Firefly luciferase is susceptible to thermal inactivation, thereby its intracellular half-life decreased. Previous reports indicated that L300R mutation (LRR mutant) in E354R/Arg356 double mutant (ERR mutant) from Lampyris turkestanicus luciferase has increased its thermal stability and rigidity through induction of some ionic bonds with Asp 270 and 271… (More)
DOI: 10.1016/j.ijbiomac.2017.03.069

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Cite this paper

@article{Yousefi2017BifunctionalRO, title={Bifunctional role of leucine 300 of firefly luciferase in structural rigidity.}, author={Farzad Yousefi and Farangis Ataei and M. Mortazavi and Saman Hosseinkhani}, journal={International journal of biological macromolecules}, year={2017}, volume={101}, pages={67-74} }