Bidder's organ in the toad Bufo marinus: Effects of orchidectomy on the morphology and expression of lamina‐associated polypeptide 2

  title={Bidder's organ in the toad Bufo marinus: Effects of orchidectomy on the morphology and expression of lamina‐associated polypeptide 2},
  author={Federico D. Brown and Eugenia M Del Pino and Georg Krohne},
The Bidder's organ is an undeveloped ovary located anterior to the testis in male true toads (Bufonidae). The presence of sperm and oocytes, derived from the primordial germ cells of the male toad, provides an exceptional condition for the study of germ cell differentiation in vertebrates. In this study, the effects of orchidectomy on morphology and on lamina‐associated polypeptide 2 (LAP2) expression were investigated in the Bidder's organ of Bufo marinus. To characterize bidderian oocytes… 
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes.
It is found that BOs weight was significantly lower in males during the pre-reproductive period and that there is no significant correlation between the weights of BO and the adjacent testis, which confirm the steroidogenic capacity of the BO in adult males of R. arenarum.
Development and steroidogenic properties of the Bidder's organ of the tadpole of Rhinella arenarum (Amphibia, Anura).
BO is able to synthesize estradiol from endogenous precursors and proper gonad remains undifferentiated at least until the end of the metamorphosis.
Molecular characterization of the Bidder's organ in the cane toad (Bufo marinus).
It is demonstrated that Sf1, Dax1, and p450arom, key genes involved in vertebrate steroidogenesis, are transcriptionally active in the BO during developmental milestones associated with sexual development and maturation, suggesting that the BO likely has a steroidogenic role in the development of the cane toad, distinct from that of the gonads.
Estradiol production by the Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Seasonal variations in plasma estradiol.
Seasonal variations in plasma E(2), in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E( 2) from endogenous substrates throughout the year are demonstrated.
Morphological and histochemical studies of Bidder’s organ in Rhinella schneideri (Amphibia: Anura) males
Specific aspects related to the morphological description of the Bidder’s organ in males of the neotropical frog Rhinella schneideri during the breeding season are described with emphasis on architecture, morphometry and histochemistry.
Morphological Aspects of Gonadal Morphogenesis in Bufo bufo (Amphibia anura): Bidder's Organ Differentiation
The asymmetric model, in which there is a tendency toward a primary female differentiation, was confirmed, and the female differentiation becomes stable in the Bidder's organ because of the absence of further interaction between germ and medullary somatic cells, which would have led toward a male differentiation.
LPS-induced alterations in reproductive organs and liver pigmentation in the toad Rhinella diptycha (Bufonidae)
The results suggest that LPS delays the release of spermatozoa, possibly due to alterations in serum levels of testosterone and (or) gonadotropins, which may also explain fewer degrading cells in Bidder’s organ.
Ultrastructural aspects of gonadal morphogenesis in Bufo bufo (Amphibia Anura) 1. Sex differentiation.
The morphogenesis of gonads in Bufo bufo tadpoles was studied, and ultrastructural differences between sexes were identified, finding that sex differentiation follows an "asymmetric" pattern in which germ cells tend primarily toward a female differentiation and male differentiation depends on a secondary interaction between germ cells and medullary somatic cells.
Bidder's organ--structure, development and function.
The development and structure of Bidder's organs are described, the knowledge on gene expression and steroidogenic activity in these organs are summarized, and hypotheses regarding Bidder’s organ function are presented.


The effects of orchidectomy and gonadotropins on steroidogenesis and oogenesis in Bidder's organs of the toad Bufo woodhousii.
The results suggest that the testes play a major role in the inhibition of oogenesis in Bidder's organs of B. woodhousii and are a major source of androgens.
The effects of aromatase and 5 alpha-reductase inhibitors, antiandrogen, and sex steroids on Bidder's organs development and gonadal differentiation in Bufo bufo tadpoles.
The strong tendency of Bidderian germ cells to develop in the oogenetic way regardless of sex genotype and steroid treatments, and the quantitative sex differences found in the control Bidder's organs and gonads suggest that other factors may be involved in the initial steps of the process of germ cell differentiation.
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals
Six stages of oocyte development in the anuran Xenopus laevis can be divided into six stages based on the anatomy of the developing oocyte, and these stages have been correlated with physiological and biochemical data related to oogenesis.
Oogenesis in Xenopus laevis (Daudin)
It is concluded that HCG acts to promote or stimulate endocytosis in developing oocytes while estrogen and/or starvation inhibits this process.
Nuclear envelope remodelling during rat spermiogenesis: distribution and expression pattern of LAP2/thymopoietins.
It is observed that during the third phase of spermatogenesis, TP-labelling shifted progressively to one half of the nuclear periphery in round sperMatids, the first demonstration that expression and localization patterns of TPs are coordinately and differentially regulated with lamins during a differentiation process.
Lamina-associated polypeptide 2 (LAP2) expression in fish and amphibians.
The differential expression of LAP2 isoforms in somatic and germ cells, as found in fish and frogs, may be ancestral vertebrate characters, and in spite of differences in developmental time, the LAP1 isoforms of somatic cells are upregulated during gastrulation, suggesting that LAP 2 may be implicated in the early development of fish and frog.
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
  • R. Benavente, KrohneG
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1985
It is hypothesize that the specific expression of lamin LIV is related to the conspicuous changes of nuclear architecture and chronmatin composition that are known to take place during the late stages of sperm development.
Germ Cells
Molecular characterization and developmentally regulated expression of Xenopus lamina-associated polypeptide 2 (XLAP2).
The results are the first description of the developmentally regulated expression of integral nuclear envelope proteins during early embryogenesis.
Axis and germ line deficiencies caused by u.v. irradiation of Xenopus oocytes cultured in vitro
A method for routinely maturing and fertilizing cultured oocytes of Xenopus is described and it is found that the u.v.-sensitive period for PGC and dorsal axis formation extends back into stage-VI oocytes, thus demonstrating a true oocyte contribution to these processes.