Bicuculline-insensitive GABA receptors: Studies on the binding of (−)-baclofen to rat cerebellar membranes

  title={Bicuculline-insensitive GABA receptors: Studies on the binding of (−)-baclofen to rat cerebellar membranes},
  author={Colleen A. Drew and Graham A. R. Johnston and Robert P. Weatherby},
  journal={Neuroscience Letters},
The binding of [3H](-)-baclofen to synaptic membranes prepared from rat cerebellum was studied. Consistent with pharmacological data that (-)-baclofen is the more active stereoisomer, studies on the binding of [3H](-)-baclofen showed increased specific binding, a higher affinity Kd and a lower Bmax than equivalent studies using [3H](+/-)-baclofen. Divalent metal ions (mercury, lead, calcium and zinc) inhibited the binding of [3H](-)-baclofen. The effects of some conformationally restricted… Expand
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