Bicarbonate Ion Stimulates the Expression of Locus of Enterocyte Effacement-Encoded Genes in Enterohemorrhagic Escherichia coli O157:H7

@article{Abe2002BicarbonateIS,
  title={Bicarbonate Ion Stimulates the Expression of Locus of Enterocyte Effacement-Encoded Genes in Enterohemorrhagic Escherichia coli O157:H7},
  author={H. Abe and I. Tatsuno and T. Tobe and A. Okutani and C. Sasakawa},
  journal={Infection and Immunity},
  year={2002},
  volume={70},
  pages={3500 - 3509}
}
ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) strains adhere to the intestinal mucosa and produce an attaching and effacing (A/E) lesion. Most of the genes required to produce A/E lesions are thought to be encoded by the 36-kb pathogenicity island termed the locus for enterocyte effacement (LEE). Although the mechanisms underlying the bacterial adherence, including the genes involved, are still poorly understood, the preferential adherence phenotype of EHEC is thought to depend on the… Expand
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References

SHOWING 1-10 OF 45 REFERENCES
The Cloned Locus of Enterocyte Effacement from EnterohemorrhagicEscherichia coli O157:H7 Is Unable To Confer the Attaching and Effacing Phenotype upon E. coliK-12
TLDR
The A/E phenotype could not be restored by trans complementation with a variety of cloned EPEC LEE fragments, suggesting functional and/or regulatory differences between the LEE pathogenicity islands of EPEC O127:H6 and EHEC O157:H7. Expand
The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69
TLDR
The entire LEE of EPEC strain E2348/69 is sequenced and the final LEE sequence entry contains corrections to some of these previously reported genes and predicted proteins and changes the name of several previously described genes comprising the type III secretion system ofEPEC. Expand
The Locus of Enterocyte Effacement (LEE)-Encoded Regulator Controls Expression of Both LEE- and Non-LEE-Encoded Virulence Factors in Enteropathogenic and EnterohemorrhagicEscherichia coli
TLDR
It is reported that Ler is essential for the expression of multiple LEE-located genes in both EPEC and EHEC, including those encoding the type III secretion pathway, the secreted Esp proteins, Tir, and intimin, which indicates that Ler regulates additional virulence properties. Expand
Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli.
TLDR
Enterohemorrhagic Escherichia coli O157:H7, which has an unusually low infectious dose, could be induced by quorum sensing of signals produced by nonpathogenic E. coli of the normal intestinal flora. Expand
Enterohemorrhagic Escherichia coliO157:H7 Produces Tir, Which Is Translocated to the Host Cell Membrane but Is Not Tyrosine Phosphorylated
TLDR
It is demonstrated that EHEC produces a functional Tir that is inserted into host cell membranes, where it serves as an intimin receptor, however, unlike in EPEC, in EH EC Tir is not tyrosine phosphorylated yet plays a key role in both bacterial adherence to epithelial cells and pedestal formation. Expand
The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)‐encoded regulator (Ler)
TLDR
The genetic organization of the LEE is determined, defining transcriptional units and mapping transcriptional start points, and components of the type III secretion system are transcribed from three polycistronic operons designated LEE1, LEE2 and LEE3. Expand
Enteropathogenic Escherichia coli protein secretion is induced in response to conditions similar to those in the gastrointestinal tract
TLDR
It is shown that the secretion of EspB is subject to environmental regulation, and maximal secretion occurs under conditions reminiscent of those in the gastrointestinal tract, and that secretion is not dependent on CO2. Expand
A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
TLDR
It is reported that in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions is found, which hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridized to avirulent members of the same species. Expand
Transcriptional Regulation of the espGenes of Enterohemorrhagic Escherichia coli
TLDR
It is determined that the genes encoding the secreted proteins EspA, EspD, and EspB of enterohemorrhagic Escherichia coli (EHEC) are organized in a single operon, and transcription of the esp operon seems to be switched off in tightly attached bacteria. Expand
The EspD Protein of EnterohemorrhagicEscherichia coli Is Required for the Formation of Bacterial Surface Appendages and Is Incorporated in the Cytoplasmic Membranes of Target Cells
TLDR
The construction and characterization of an in-frame espD deletion mutant of the enterohemorrhagic E. coli (EHEC) strain EDL933 is reported, which not only lacked the capacity to accumulate actin but also exhibited an impaired attachment to HeLa cells. Expand
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