Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure

@inproceedings{Fuchs2015BiasIL,
  title={Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure},
  author={Ryan T. Fuchs and Zhiyi Sun and Fanglei Zhuang and Gregory B. Robb},
  booktitle={PloS one},
  year={2015}
}
High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs in a sample has been shown to be inconsistent with quantitative PCR and Northern Blot results. Several recent studies have concluded that the major contributor to this inconsistency is bias introduced during the construction of sRNA libraries for… CONTINUE READING
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