BglBrick vectors and datasheets: A synthetic biology platform for gene expression

  title={BglBrick vectors and datasheets: A synthetic biology platform for gene expression},
  author={Taek S Lee and Rachel A Krupa and Fuzhong Zhang and Meghdad Hajimorad and William J. Holtz and Nilu Prasad and Sung Kuk Lee and Jay D Keasling},
  booktitle={Journal of biological engineering},
BACKGROUND As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such systems often requires screening combinations of origins of replication, expression systems, and antibiotic markers. This procedure is hampered by a lack of quantitative data on how these components behave when more than one origin of replication or expression system are used… CONTINUE READING
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