Beyond self-assembly: From microtubules to morphogenesis

  title={Beyond self-assembly: From microtubules to morphogenesis},
  author={Marc W. Kirschner and Timothy J. Mitchison},

Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools

Live imaging is used to reveal molecular basis of the interaction between chromosomes and microtubules particularly important for oocytes, which may contribute to generation of force pushing chromosomes toward spindle equator.

Search-and-capture model of cytoneme-mediated morphogen gradient formation.

A search-and-capture model of cytoneme-based morphogenesis, in which nucleating cytonemes from a source cell dynamically grow and shrink along the surface of a one-dimensional array of target cells until making contact with one of the target cells, is developed.

The role of chromosome arms and kinetochore architecture in the formation and amphitelic attachments - implications for mitotic fidelity

It is shown that conversion from lateral to end-on kinetochore-microtubule attachments was promoted by polar ejection forces (PEFs) generated by Chromokinesins ultimately leading to SAC satisfaction in SMUGs, providing direct evidence that kinetchore size is an important determinant of chromosome segregation fidelity in mammals.

Kinetochore-derived microtubules : from molecular regulation to their role in mitosis

This work has shown that budding yeast S. cerevisiae is a good model organism to study the role of kinetochore-derived microtubules in mitosis and provides a platform for microtubule nucleation at kinetchores ............................................. 115 2.

Intrinsic microtubule GTP-cap dynamics in semi-confined systems: kinetochore–microtubule interface

The proposed novel quantitative concept of critical nano local GTP-tubulin concentration (CNLC) enables the formulation of a rationale to explain the intrinsic nature of the “push-and-pull” mechanism associated with a kinetochore–microtubule complex.

Comparative autoregressive moving average analysis of kinetochore microtubule dynamics in yeast.

To elucidate the regulation of kinetochore microtubules (kMTs) by kinetochore proteins in Saccharomyces cerevisiae, we need tools to characterize and compare stochastic kMT dynamics. Here we show

E pluribus unum: towards a universal mechanism for spindle assembly.

Kinetochore–microtubule interactions during cell division

The history of research devoted to the study of kinetochore–microtubule interaction is looked at and significant advances in the knowledge of the basic processes are attempted to identify.

Dynein is required for spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes.

It is proposed that dynein function is required for the association of chromosomes with astral microtubules during in vitro meiosis I spindle assembly in these lysate-extract mixtures, but it is concluded that Dyneinfunction is not required for centrosome assembly and maturation or for Centrosome-dependent aster formation.



The Biochemistry of Microtubules

The whole of the complex field of microtubule structure and function is far too broad to cover in this brief review. The intention has been to give a brief overview of the field and to place emphasis

Regulation of Structural Protein Interactions as Revealed in Phage Morphogenesis

Advances in cellular and molecular biology continue to reveal new aspects of the structural organization within cells that differ in many ways from the classical models for the control of intermediary metabolism, such as the induction and repression of enzyme synthesis.

In vitro assembly of cytoplasmic microtubules.

A comparison study of the Nucleated Polymerization Model and its applications in the assembly process finds that T and HMW-MAPs specijic is a viable alternative for HMW fraction fractionation.