Beyond natural antibodies: the power of in vitro display technologies

@article{Bradbury2011BeyondNA,
  title={Beyond natural antibodies: the power of in vitro display technologies},
  author={A. Bradbury and S. Sidhu and S. D{\"u}bel and J. McCafferty},
  journal={Nature biotechnology},
  year={2011},
  volume={29},
  pages={245 - 254}
}
In vitro display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using in vitro display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using in vitro display methods… Expand

Figures, Tables, and Topics from this paper

Selection of Antibody Fragments by Yeast Display.
  • N. Scholler
  • Biology, Medicine
  • Methods in molecular biology
  • 2018
TLDR
This chapter gives an updated overview of the available systems of yeast display platforms and libraries, followed up by technical descriptions of selection methods of antibody fragments by yeast display. Expand
Selection of antibody fragments by yeast display.
TLDR
This chapter gives an updated overview of the available systems of yeast display platforms and libraries, followed up by technical descriptions of selection methods of antibody fragments by yeast display. Expand
Mammalian cell display for the discovery and optimization of antibody therapeutics.
TLDR
The display system has been developed to enable simultaneous high-level cell surface expression and secretion of the same protein through alternate splicing, where the displayed protein phenotype remains linked to genotype, allowing soluble secreted antibody to be simultaneously characterized in biophysical and cell-based functional assays. Expand
Antibody display technologies: selecting the cream of the crop
TLDR
This review delineates the most important selection systems with respect to antibody generation with an emphasis on recent developments. Expand
Selection of recombinant antibodies from antibody gene libraries.
TLDR
Detailed protocols are given for the selection of recombinant antibody fragments from antibody gene libraries in microtiter plates based on the specific molecular interaction of antibody phage with an immobilized antigen thus allowing the enrichment and isolation of antigen-specific monoclonal binders from very large antibody Gene libraries. Expand
Designing Human Antibodies by Phage Display
TLDR
Some opportunities and achievements are summarized, e.g., the generation of antibodies which could not be generated otherwise, and the design of antibody properties by different panning strategies, including the adjustment of kinetic parameters. Expand
High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires
TLDR
This chapter will focus on the technologies commonly applied in antibody display technologies to engineer improved affinities. Expand
Mammalian cell display technology coupling with AID induced SHM in vitro: an ideal approach to the production of therapeutic antibodies.
TLDR
This review was concentrated on the development of the mammalian cell display technology as well as the activation-induced cytidine deaminase induced in vitro somatic hypermutation technology and their applications for the production of therapeutic antibodies. Expand
Drugs derived from phage display
TLDR
This review uses case studies, from candidate identification through clinical development, to illustrate the utility ofphage display as a drug discovery tool, and offers a perspective for future developments of phage display technology. Expand
Engineering Antibodies on the Surface of CHO Cells.
TLDR
A Chinese hamster ovary (CHO) cell-based selection system that allows for long-term display of antibody Fab fragments and the simple and accessible use of CHO display coupled with flow cytometry to enrich for antibody variants with increased ligand-binding affinity from large libraries of ~106 variants. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 190 REFERENCES
Application of phage display to high throughput antibody generation and characterization
TLDR
The potential of and the issues associated with genome-wide monoclonal antibody generation are demonstrated and illuminated by a high quality phage display library containing over 1010 human antibodies. Expand
Generating recombinant antibodies to the complete human proteome.
TLDR
This opinion article summarizes opportunities for the generation of antibodies for proteome research using in vitro technologies by noting the handling, transport and storage logistics and bacterial production offer cost benefits. Expand
Isolating and engineering human antibodies using yeast surface display
TLDR
This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display using magnetic-activated cell sorting selection and flow cytometry to attain desired scFv properties through directed evolution. Expand
Design and Use of a Phage Display Library
TLDR
A large repertoire of functional antibodies with similar properties was produced by appending short variable complementarity-determining region 3 (CDR3) onto the two antibody germ line segments most frequently found in human antibodies by concentrating sequence diversity in regions of the antibody structure that are centrally located in the antigen binding site. Expand
Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display
TLDR
This work has mimicked the process of antibody generation and affinity maturation with a synthetic library in a cell-free system in just a few days, obtaining molecules with higher affinities than most natural antibodies. Expand
Phage antibodies: filamentous phage displaying antibody variable domains
TLDR
It is shown that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage can be isolated after affinity chromatography. Expand
Selection of antibodies for intracellular function using a two-hybrid in vivo system.
TLDR
It is found that several characterized antibodies can bind their target antigen in eukaryotic cells in this two-hybrid format, and the approach can provide the basis for de novo selection of intracellular scFv from libraries, such as those made from spleen RNA after immunization with antigen, for intrACEllular analysis of protein function based only on genomic or cDNA sequences. Expand
Molecular engineering and design of therapeutic antibodies.
  • L. Presta
  • Biology, Medicine
  • Current opinion in immunology
  • 2008
TLDR
Over the past two years significant progress in designing antibodies with improved pharmacokinetic properties, via modified interaction with the neonatal Fc receptor (FcRn), has been achieved and the ability to alter the communication of a therapeutic antibody with the immune system has been advanced. Expand
From rodent reagents to human therapeutics using antibody guided selection.
TLDR
Guided selection has successfully been used for the generation of a number of human versions of rodent antibodies, including HUMIRA, an inhibitor of tumour necrosis factor-alpha which is approved for the treatment of moderate to severe rheumatoid arthritis in over 40 countries. Expand
Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens.
TLDR
This work used yeast-displayed antigens known to be associated with a cell type to select the phage antibody output after several rounds of selection on a mammalian cell line to generate several human phage antibodies to yeast- displayed EphA2 and CD44. Expand
...
1
2
3
4
5
...