Beware of proteins in DMSO.

  title={Beware of proteins in DMSO.},
  author={Michael F Jackson and Henry H. Mantsch},
  journal={Biochimica et biophysica acta},
  volume={1078 2},
The effect on the secondary structure of representative alpha-helical, beta-sheet and disordered proteins by varying concentrations of dimethyl sulphoxide (DMSO) in 2H2O has been investigated by Fourier transform infrared spectroscopy. Significant perturbations of protein secondary structure are induced by DMSO and DMSO/2H2O mixtures. For highly structured proteins, such as myoglobin and concanavalin A, the infrared spectra point to a progressive destabilisation of the secondary structure until… CONTINUE READING