Beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing.

@article{Makabe2007BetastrandFA,
  title={Beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing.},
  author={Koki Makabe and Shude Yan and Valentina Tereshko and Grzegorz Gawlak and Shohei Koide},
  journal={Journal of the American Chemical Society},
  year={2007},
  volume={129 47},
  pages={14661-9}
}
We investigated how the register between adjacent beta-strands is specified using a series of mutants of the single-layer beta-sheet (SLB) in Borrelia OspA. The single-layer architecture of this system eliminates structural restraints imposed by a hydrophobic core, enabling us to address this question. A critical turn (turn 9/10) in the SLB was replaced with a segment with an intentional structural mismatch. Its crystal structure revealed a one-residue insertion into the central beta-strand… CONTINUE READING