Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana.

@article{Wang2008BetalactonePI,
  title={Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana.},
  author={Zhemin Wang and C. Gu and T. Colby and T. Shindo and R. Balamurugan and H. Waldmann and M. Kaiser and R. V. D. van der Hoorn},
  journal={Nature chemical biology},
  year={2008},
  volume={4 9},
  pages={
          557-63
        }
}
New activity-based probes are essential for expanding studies on the hundreds of serine and cysteine proteases encoded by the genome of Arabidopsis thaliana. To monitor protease activities in plant extracts, we generated biotinylated peptides containing a beta-lactone reactive group. These probes cause strong labeling in leaf proteomes. Unexpectedly, labeling was detected at the N terminus of PsbP, nonproteolytic protein of photosystem II. Inhibitor studies and reverse genetics led to the… Expand
Probes for activity-based profiling of plant proteases.
Activity-based protein profiling in plants
Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves
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